Structural organization of the membrane-bound hydrogenase isolated from Alcaligenes eutrophus as revealed by electron microscopy
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چکیده
منابع مشابه
The membrane-bound hydrogenase of Alcaligenes eutrophus. I. Solubilization, purification, and biochemical properties.
The membrane-bound hydrogenase of Alcaligenes eutrophus was solubilized from washed membranes of autotrophically grown cells. The enzyme consists of two types of subunits and is an iron-sulfur protein. A flavin compound was not detected. The enzyme reacts only with few artificial electron acceptors.
متن کاملAntigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm.
Electron microscopic immunogold labeling experiments were performed with ultrathin sections of plasmolyzed cells of Alcaligenes eutrophus and "whole-mount" samples of spheroplasts and protoplasts. They demonstrated that antigenic determinants of the membrane-bound hydrogenase are exposed, at the outside of the cytoplasmic membrane, to the periplasm.
متن کاملThe Alcaligenes eutrophus H16 hoxX gene participates in hydrogenase regulation.
Nucleotide sequence analysis revealed a 1,791-bp open reading frame in the hox gene cluster of the gram-negative chemolithotroph Alcaligenes eutrophus H16. In order to investigate the biological role of this open reading frame, we generated an in-frame deletion allele via a gene replacement strategy. The resulting mutant grew significantly more slowly than the wild type under lithoautotrophic c...
متن کاملNickel requirement for active hydrogenase formation in Alcaligenes eutrophus.
The nickel-dependent chemolithoautotrophic growth of Alcaligenes eutrophus is apparently due to a requirement of nickel for active hydrogenase formation. Cells grown heterotrophically with fructose and glycerol revealed a specific activity of soluble and membrane-bound hydrogenase which was severalfold higher than the normal autotrophic level. The omission of nickel from the medium did not affe...
متن کاملExploring Membrane Microdomain Organization by Electron Microscopy
The flow of information through cells requires the constant remodeling of cell signaling and trafficking networks. To observe the remodeling events associated with receptor activation, we have generated and analyzed high resolution topographical maps of colloidal gold nanoprobes marking receptors and signaling proteins in native membranes. The technology involves labeling membrane sheets stripp...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1989
ISSN: 0378-1097
DOI: 10.1016/0378-1097(89)90500-4